Norman Lab

Publications

Highlighted

Mapping information-rich genotype-phenotype landscapes with genome-scale Perturb-seq
Mapping information-rich genotype-phenotype landscapes with genome-scale Perturb-seq
Joseph M. Replogle, Reuben A. Saunders, Angela N. Pogson, Jeffrey A. Hussmann, Alexander Lenail, …, Doron Lipson, Jessica L. Bonnar, Marco Jost, Thomas M. Norman*, Jonathan S. Weissman*
Cell  ·  01 Jul 2022  ·  doi:10.1016/j.cell.2022.05.013
Source
Exploring genetic interaction manifolds constructed from rich single-cell phenotypes
Exploring genetic interaction manifolds constructed from rich single-cell phenotypes
Thomas M. Norman*, Max A. Horlbeck*, Joseph M. Replogle, Alex Y. Ge, Albert Xu, Marco Jost, Luke A. Gilbert, Jonathan S. Weissman
Science  ·  23 Aug 2019  ·  doi:10.1126/science.aax4438
Source
A Multiplexed Single-Cell CRISPR Screening Platform Enables Systematic Dissection of the Unfolded Protein Response
A Multiplexed Single-Cell CRISPR Screening Platform Enables Systematic Dissection of the Unfolded Protein Response
Britt Adamson*, Thomas M. Norman*, Marco Jost, Min Y. Cho, James K. Nuñez, …, Carol A. Gross, Atray Dixit, Oren Parnas, Aviv Regev, Jonathan S. Weissman
Cell  ·  01 Dec 2016  ·  doi:10.1016/j.cell.2016.11.048
Source

All

2024

Comprehensive transcription factor perturbations recapitulate fibroblast transcriptional states
Comprehensive transcription factor perturbations recapitulate fibroblast transcriptional states
Kaden M Southard, Rico C. Ardy, Anran Tang, Deirdre D. O’Sullivan, Eli Metzner, Karthik Guruvayurappan, Thomas M. Norman
bioRxiv  ·  03 Aug 2024  ·  doi:10.1101/2024.07.31.606073
Summary
Multiome Perturb-seq unlocks scalable discovery of integrated perturbation effects on the transcriptome and epigenome
Multiome Perturb-seq unlocks scalable discovery of integrated perturbation effects on the transcriptome and epigenome
Eli Metzner, Kaden M. Southard, Thomas M. Norman
bioRxiv  ·  27 Jul 2024  ·  doi:10.1101/2024.07.26.605307
Summary

2023

Drivers of heterogeneity in synovial fibroblasts in rheumatoid arthritis
Drivers of heterogeneity in synovial fibroblasts in rheumatoid arthritis
Melanie H. Smith, Vianne R. Gao, Preethi K. Periyakoil, Alejandro Kochen, Edward F. DiCarlo, Susan M. Goodman, Thomas M. Norman, Laura T. Donlin, Christina S. Leslie, Alexander Y. Rudensky
Nature Immunology  ·  05 Jun 2023  ·  doi:10.1038/s41590-023-01527-9

2022

Mapping information-rich genotype-phenotype landscapes with genome-scale Perturb-seq
Mapping information-rich genotype-phenotype landscapes with genome-scale Perturb-seq
Joseph M. Replogle, Reuben A. Saunders, Angela N. Pogson, Jeffrey A. Hussmann, Alexander Lenail, …, Doron Lipson, Jessica L. Bonnar, Marco Jost, Thomas M. Norman*, Jonathan S. Weissman*
Cell  ·  01 Jul 2022  ·  doi:10.1016/j.cell.2022.05.013
Source
High-content CRISPR screening
High-content CRISPR screening
Christoph Bock, Paul Datlinger, Florence Chardon, Matthew A. Coelho, Matthew B. Dong, …, Lei S. Qi, Rebecca S. Shapiro, Jay Shendure, Jonathan S. Weissman, Xiaowei Zhuang
Nature Reviews Methods Primers  ·  10 Feb 2022  ·  doi:10.1038/s43586-021-00093-4

2020

Combinatorial single-cell CRISPR screens by direct guide RNA capture and targeted sequencing
Combinatorial single-cell CRISPR screens by direct guide RNA capture and targeted sequencing
Joseph M. Replogle, Thomas M. Norman, Albert Xu, Jeffrey A. Hussmann, Jin Chen, …, Luigi J. Alvarado, Katherine A. Pfeiffer, Tarjei S. Mikkelsen, Jonathan S. Weissman, Britt Adamson
Nature Biotechnology  ·  30 Mar 2020  ·  doi:10.1038/s41587-020-0470-y
Titrating gene expression using libraries of systematically attenuated CRISPR guide RNAs
Titrating gene expression using libraries of systematically attenuated CRISPR guide RNAs
Marco Jost, Daniel A. Santos, Reuben A. Saunders, Max A. Horlbeck, John S. Hawkins, …, Thomas M. Norman, Jeffrey A. Hussmann, Christina R. Liem, Carol A. Gross, Jonathan S. Weissman
Nature Biotechnology  ·  13 Jan 2020  ·  doi:10.1038/s41587-019-0387-5

2019

Stochastic antagonism between two proteins governs a bacterial cell fate switch
Stochastic antagonism between two proteins governs a bacterial cell fate switch
Nathan D. Lord, Thomas M. Norman, Ruoshi Yuan, Somenath Bakshi, Richard Losick, Johan Paulsson
Science  ·  04 Oct 2019  ·  doi:10.1126/science.aaw4506
Exploring genetic interaction manifolds constructed from rich single-cell phenotypes
Exploring genetic interaction manifolds constructed from rich single-cell phenotypes
Thomas M. Norman*, Max A. Horlbeck*, Joseph M. Replogle, Alex Y. Ge, Albert Xu, Marco Jost, Luke A. Gilbert, Jonathan S. Weissman
Science  ·  23 Aug 2019  ·  doi:10.1126/science.aax4438
Source
Molecular recording of mammalian embryogenesis
Molecular recording of mammalian embryogenesis
Michelle M. Chan, Zachary D. Smith, Stefanie Grosswendt, Helene Kretzmer, Thomas M. Norman, …, Matthew G. Jones, Alex Khodaverdian, Nir Yosef, Alexander Meissner, Jonathan S. Weissman
Nature  ·  13 May 2019  ·  doi:10.1038/s41586-019-1184-5

2016

Perturb-Seq: Dissecting Molecular Circuits with Scalable Single-Cell RNA Profiling of Pooled Genetic Screens
Perturb-Seq: Dissecting Molecular Circuits with Scalable Single-Cell RNA Profiling of Pooled Genetic Screens
Atray Dixit, Oren Parnas, Biyu Li, Jenny Chen, Charles P. Fulco, …, Thomas M. Norman, Eric S. Lander, Jonathan S. Weissman, Nir Friedman, Aviv Regev
Cell  ·  01 Dec 2016  ·  doi:10.1016/j.cell.2016.11.038
A Multiplexed Single-Cell CRISPR Screening Platform Enables Systematic Dissection of the Unfolded Protein Response
A Multiplexed Single-Cell CRISPR Screening Platform Enables Systematic Dissection of the Unfolded Protein Response
Britt Adamson*, Thomas M. Norman*, Marco Jost, Min Y. Cho, James K. Nuñez, …, Carol A. Gross, Atray Dixit, Oren Parnas, Aviv Regev, Jonathan S. Weissman
Cell  ·  01 Dec 2016  ·  doi:10.1016/j.cell.2016.11.048
Source
Exploiting Natural Fluctuations to Identify Kinetic Mechanisms in Sparsely Characterized Systems
Exploiting Natural Fluctuations to Identify Kinetic Mechanisms in Sparsely Characterized Systems
Andreas Hilfinger, Thomas M. Norman, Johan Paulsson
Cell Systems  ·  01 Apr 2016  ·  doi:10.1016/j.cels.2016.04.002
Constraints on Fluctuations in Sparsely Characterized Biological Systems
Constraints on Fluctuations in Sparsely Characterized Biological Systems
Andreas Hilfinger, Thomas M. Norman, Glenn Vinnicombe, Johan Paulsson
Physical Review Letters  ·  01 Feb 2016  ·  doi:10.1103/PhysRevLett.116.058101

2013

Memory and modularity in cell-fate decision making
Memory and modularity in cell-fate decision making
Thomas M. Norman, Nathan D. Lord, Johan Paulsson, Richard Losick
Nature  ·  01 Nov 2013  ·  doi:10.1038/nature12804

2011

Evidence that metabolism and chromosome copy number control mutually exclusive cell fates in<i>Bacillus subtilis</i>
Evidence that metabolism and chromosome copy number control mutually exclusive cell fates inBacillus subtilis
Yunrong Chai, Thomas Norman, Roberto Kolter, Richard Losick
The EMBO Journal  ·  15 Feb 2011  ·  doi:10.1038/emboj.2011.36

2010

Broadly heterogeneous activation of the master regulator for sporulation in <i>Bacillus subtilis</i>
Broadly heterogeneous activation of the master regulator for sporulation in Bacillus subtilis
Arnaud Chastanet, Dennis Vitkup, Guo-Cheng Yuan, Thomas M. Norman, Jun S. Liu, Richard M. Losick
Proceedings of the National Academy of Sciences  ·  19 Apr 2010  ·  doi:10.1073/pnas.1002499107
An epigenetic switch governing daughter cell separation in <i>Bacillus subtilis</i>
An epigenetic switch governing daughter cell separation in Bacillus subtilis
Yunrong Chai, Thomas Norman, Roberto Kolter, Richard Losick
Genes & Development  ·  29 Mar 2010  ·  doi:10.1101/gad.1915010