Norman Lab

The Norman Lab aims to combine large-scale functional genomics experiments with computational modeling to enable rational engineering of cell state. Our primary model system is the fibroblast, a ubiquitous and phenotypically plastic cell type that plays a role in diverse pathologies. We played a foundational role in developing the Perturb-seq approach for single-cell CRISPR screening and continue to develop new approaches to enable functional genomics experiments beyond genome-scale. Our ultimate goal is to learn principles for precisely controlling cellular behavior, with potential applications in cell therapy and disease modeling.

Genome-scale Perturb-seq experiments knocking down ~8,000 transcripts in K562 cells. Transcriptome phenotypes formed 64 biologically meaningful clusters, with purple labels indicating recapitulated CORUM protein complexes.
Genome-scale Perturb-seq experiments knocking down ~8,000 transcripts in K562 cells. Transcriptome phenotypes formed 64 biologically meaningful clusters, with purple labels indicating recapitulated CORUM protein complexes.

Updates

January 2025 Multiome Perturb-seq paper is out in Cell Systems. Processed data here. Protocol here.

August 2024 Kaden and Rico’s preprint on recreating fibroblast transcriptional states using CRISPRa Perturb-seq is out! Overview here.

July 2024 Eli and Kaden’s preprint on Multiome Perturb-seq is out! Overview here.

July 2022 Genomewide Perturb-seq, joint with Weissman Lab, is out in Cell! Raw and processed data available here.